The depuration dynamics of oysters ( Crassostrea gigas ) artificially contaminated with hepatitis A virus and human adenovirus

Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral path...

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Published inMemórias do Instituto Oswaldo Cruz Vol. 107; no. 1; pp. 11 - 17
Main Authors Corrêa, Adriana de Abreu, Rigotto, Caroline, Moresco, Vanessa, Kleemann, Cristian Rafael, Teixeira, Adriano Luiz, Poli, Carlos Rogério, Simões, Clàudia Maria Oliveira, Barardi, Célia Regina Monte
Format Journal Article
LanguageEnglish
Portuguese
Published Brazil Fundação Oswaldo Cruz, Fiocruz 01.02.2012
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects
Adenoviruses, Human - radiation effects
Animals
aquaculture
Aquaculture - methods
Crassostrea - virology
depuration
Disinfection - methods
Dose-Response Relationship, Radiation
Food Microbiology
food safety
Hepatitis A virus - radiation effects
PARASITOLOGY
Polymerase Chain Reaction
Seawater - virology
shellfish
Time Factors
TROPICAL MEDICINE
Ultraviolet Rays
viruses
food safety
aquaculture
shellfish
viruses
depuration
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Abstract Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV.
AbstractList Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV.
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral pathogens from artificially infected oysters and analysed the results. Specifically, the oysters were contaminated with hepatitis A virus (HAV) or human adenovirus type 5 (HAdV5). After viral infection, the oysters were placed into a depuration tank and harvested after 48, 72 and 96 h. After sampling, various oyster tissues were dissected and homogenised and the viruses were eluted with alkaline conditions and precipitated with polyethylene glycol. The oyster samples were evaluated by cell culture methods, as well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover, at the end of the depuration period, the disinfected seawater was collected and analysed by PCR. The molecular assays showed that the HAdV5 genome was present in all of the depuration time samples, while the HAV genome was undetectable after 72 h of depuration. However, viral viability tests (integrated cell culture-PCR and immunofluorescence assay) indicated that both viruses were inactivated with 96 h of seawater recirculation. In conclusion, after 96 h of UV treatment, the depuration system studied in this work purified oysters that were artificially contaminated with HAdV5 and HAV.
Author Corrêa, Adriana de Abreu
Simões, Clàudia Maria Oliveira
Moresco, Vanessa
Kleemann, Cristian Rafael
Rigotto, Caroline
Teixeira, Adriano Luiz
Poli, Carlos Rogério
Barardi, Célia Regina Monte
AuthorAffiliation Universidade Federal de Santa Catarina
Blue Water Aquaculture Ltda
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  fullname: Rigotto, Caroline
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  fullname: Kleemann, Cristian Rafael
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  fullname: Teixeira, Adriano Luiz
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  fullname: Poli, Carlos Rogério
– sequence: 7
  fullname: Simões, Clàudia Maria Oliveira
– sequence: 8
  fullname: Barardi, Célia Regina Monte
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Issue 1
Keywords food safety
aquaculture
shellfish
viruses
depuration
Language English
Portuguese
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Snippet Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality...
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality...
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SubjectTerms Adenoviruses, Human - radiation effects
Animals
aquaculture
Aquaculture - methods
Crassostrea - virology
depuration
Disinfection - methods
Dose-Response Relationship, Radiation
Food Microbiology
food safety
Hepatitis A virus - radiation effects
PARASITOLOGY
Polymerase Chain Reaction
Seawater - virology
shellfish
Time Factors
TROPICAL MEDICINE
Ultraviolet Rays
viruses
Title The depuration dynamics of oysters ( Crassostrea gigas ) artificially contaminated with hepatitis A virus and human adenovirus
URI http://www.bioline.org.br/abstract?id=oc12002
https://www.ncbi.nlm.nih.gov/pubmed/22310530
https://search.proquest.com/docview/920369070
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https://doaj.org/article/217921f15740417cb971ee9c577109e2
Volume 107
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