The depuration dynamics of oysters ( Crassostrea gigas ) artificially contaminated with hepatitis A virus and human adenovirus
Within the country of Brazil, Santa Catarina is a major shellfish producer. Detection of viral contamination is an important step to ensure production quality and consumer safety during this process. In this study, we used a depuration system and ultraviolet (UV) disinfection to eliminate viral path...
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Published in | Memórias do Instituto Oswaldo Cruz Vol. 107; no. 1; pp. 11 - 17 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English Portuguese |
Published |
Brazil
Fundação Oswaldo Cruz, Fiocruz
01.02.2012
Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ) |
Subjects | |
Online Access | Get full text |
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Summary: | Within the country of Brazil, Santa Catarina is a major shellfish
producer. Detection of viral contamination is an important step to
ensure production quality and consumer safety during this process. In
this study, we used a depuration system and ultraviolet (UV)
disinfection to eliminate viral pathogens from artificially infected
oysters and analysed the results. Specifically, the oysters were
contaminated with hepatitis A virus (HAV) or human adenovirus type 5
(HAdV5). After viral infection, the oysters were placed into a
depuration tank and harvested after 48, 72 and 96 h. After sampling,
various oyster tissues were dissected and homogenised and the viruses
were eluted with alkaline conditions and precipitated with polyethylene
glycol. The oyster samples were evaluated by cell culture methods, as
well as polymerase chain reaction (PCR) and quantitative-PCR. Moreover,
at the end of the depuration period, the disinfected seawater was
collected and analysed by PCR. The molecular assays showed that the
HAdV5 genome was present in all of the depuration time samples, while
the HAV genome was undetectable after 72 h of depuration. However,
viral viability tests (integrated cell culture-PCR and
immunofluorescence assay) indicated that both viruses were inactivated
with 96 h of seawater recirculation. In conclusion, after 96 h of UV
treatment, the depuration system studied in this work purified oysters
that were artificially contaminated with HAdV5 and HAV. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1678-8060 0074-0276 1678-8060 0074-0276 |
DOI: | 10.1590/S0074-02762012000100002 |