Saccharomyces boulardii upgrades cellular adaptation after proximal enterectomy in rats

• Published in: Gut Vol. 45; no. 1; pp. 89 - 96
• Main Authors: Buts, J-P, De Keyser, N, Marandi, S, Hermans, D, Sokal, E M, Chae, Y-H E, Lambotte, L, Chanteux, H, Tulkens, P M
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and British Society of Gastroenterology 01.07.1999; BMJ; BMJ Publishing Group LTD
• Subjects: Adaptation; Adaptation, Physiological; Animals; Biological and medical sciences; brush border membrane enzymes; Cell division; Digestive system; Disaccharidases - metabolism; Enzymes; Food; glucose uptake; Ileum - enzymology; Ileum - microbiology; Ileum - physiopathology; Ileum - surgery; intestine; Intubation; Laboratories; Male; Medical sciences; Pharmacology. Drug treatments; Polyamines; Polyamines - metabolism; Postoperative Care - methods; Proteins; Rats; Rats, Wistar; Rodents; Saccharomyces; Saccharomyces boulardii; Small intestine; Sodium-Potassium-Exchanging ATPase - metabolism; Surgery; Probiotic; Rat; Digestive system; Rodentia; Gut; Adaptive response; Surgical resection; Experimental study; Ileum; Vertebrata; Mammalia; Surgery; Animal
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.45.1.89

BACKGROUND Saccharomyces boulardii is a non-pathogenic yeast which exerts trophic effects on human and rat small intestinal mucosa. AIMS To examine the effects of S boulardii on ileal adaptation after proximal enterectomy in rats. METHODS Wistar rats, aged eight weeks, underwent 60% proximal resection or transection and received by orogastric intubation either 1 mg/g body wt per day lyophilised S boulardii or the vehicle for seven days. The effects on ileal mucosal adaptation were assessed eight days after surgery. RESULTS Compared with transection, resection resulted in mucosal hyperplasia with significant decreases in the specific and total activities of sucrase, lactase, and maltase. Treatment of resected animals with S boulardii had no effect on mucosal hyperplasia but did upgrade disaccharidase activities to the levels of the transected group. Enzyme stimulation by S boulardii was associated with significant increases in diamine oxidase activity and mucosal polyamine concentrations. Likewise, sodium dependentd-glucose uptake by brush border membrane vesicles, measured as a function of time and glucose concentration in the incubation medium, was significantly (p<0.05) increased by 81% and three times respectively in the resected group treated withS boulardii. In agreement with this, expression of the sodium/glucose cotransporter-1 in brush border membranes of resected rats treated with S boulardii was enhanced twofold compared with resected controls. CONCLUSION Oral administration of S boulardii soon after proximal enterectomy improves functional adaptation of the remnant ileum.