A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

• Published in: BMC research notes Vol. 2; no. 1; p. 63
• Main Authors: Kärkkäinen, Hanna-Riikka, Lesch, Hanna P, Määttä, Antti I, Toivanen, Pyry I, Mähönen, Anssi J, Roschier, Miia M, Airenne, Kari J, Laitinen, Olli H, Ylä-Herttuala, Seppo
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 23.04.2009; BioMed Central; BMC
• Subjects: Baculovirus; Baculoviruses; Cells; Gene expression; Physiological aspects; Recombinant proteins; Technical Note

Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. We describe here protocols which adapt baculovirus generation into 96-well format. The established methodology allows simple baculovirus...