A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. We describe here protocols which adapt baculovirus generation into 96-well format. The established methodology allows simple baculovirus...

Full description

Saved in:
Bibliographic Details
Published inBMC research notes Vol. 2; no. 1; p. 63
Main Authors Kärkkäinen, Hanna-Riikka, Lesch, Hanna P, Määttä, Antti I, Toivanen, Pyry I, Mähönen, Anssi J, Roschier, Miia M, Airenne, Kari J, Laitinen, Olli H, Ylä-Herttuala, Seppo
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 23.04.2009
BioMed Central
BMC
Subjects
Baculovirus
Baculoviruses
Cells
Gene expression
Physiological aspects
Recombinant proteins
Technical Note
Online AccessGet full text

Cover

More Information
Summary:Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications. We describe here protocols which adapt baculovirus generation into 96-well format. The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells in vitro and in vivo.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1756-0500
1756-0500
DOI:10.1186/1756-0500-2-63