Immunoreactive somatostatin in the rat ovary

Immunoreactive SRIH was detected in the rat ovary (15.6 pg/mg wet weight, 520 pg/mg protein) and was localized to the granulosa cells (168±6 pg/106 cells). Serial dilution studies showed parallelism of the inhibition curve for synthetic SRIH-14 and those of extracts of whole ovary and media conditio...

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Published inActa endocrinologica (Copenhagen) Vol. 126; no. 6; pp. 553 - 558
Main Authors McIntyre, H D, Maréchal, D J, Déby, G P, Mathieu, A G, Hézée-Hagelstein, M T, Franchimont, P P
Format Journal Article
LanguageEnglish
Published Denmark 01.06.1992
Subjects
Animals
Blotting, Northern
Cells, Cultured
Chorionic Gonadotropin - pharmacology
Culture Media
Female
Follicle Stimulating Hormone - pharmacology
Gene Expression
Granulosa Cells - chemistry
Granulosa Cells - drug effects
Granulosa Cells - metabolism
Meiosis - drug effects
Oocytes - cytology
Oocytes - drug effects
Ovary - chemistry
Polymerase Chain Reaction
Rats
Rats, Inbred Strains
RNA - analysis
Somatostatin - analysis
Somatostatin - genetics
Somatostatin - metabolism
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Summary:Immunoreactive SRIH was detected in the rat ovary (15.6 pg/mg wet weight, 520 pg/mg protein) and was localized to the granulosa cells (168±6 pg/106 cells). Serial dilution studies showed parallelism of the inhibition curve for synthetic SRIH-14 and those of extracts of whole ovary and media conditioned by granulosa cells. The quantity of immunoreactive SRIH released into granulosa cell conditioned media decreased with time, while the intracellular content remained relatively constant. Gel chromatography showed peaks of immunoreactivity co-eluting with SRIH-14 (38%), SRIH-28 (31%) and a high molecular weight component. The addition of synthetic SRIH-14 stimulated meiotic maturation in cumulus-enclosed rat oocytes, with dose dependency being observed at SRIH-14 concentrations between 1 and 1000 pmol/l. No evidence of pre-pro-SRIH gene expression could be demonstrated in either rat ovary or testis using both Northern analysis and reverse transcriptase/polymerase chain reaction amplification of polyadenylated RNA. SRIH may be produced in the ovary during a specific stage of ontogeny or by an alternative gene. It is also possible that SRIH is actively taken up and stored by granulosa cells without being produced locally.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.1260553