Enhancement of ligninolytic enzyme activities in a Trametes maxima - Paecilomyces carneus co-culture: Key factors revealed after screening using a Plackett-Burman experimental design
Background: In the industrial biotechnology, ligninolytic enzymes are produced by single fungal strains. Experimental evidence suggests that co-culture of ligninolytic fungi and filamentous microfungi results in an increase laccase activity. In this topic, only the ascomycete Trichoderma spp. has be...
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Published in | Electronic Journal of Biotechnology Vol. 17; no. 3; pp. 114 - 121 |
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Main Authors | , , , |
Format | Journal Article |
Language | English Portuguese |
Published |
Chile
Universidad Católica de Valparaíso
01.05.2014
Elsevier B.V Pontificia Universidad Católica de Valparaíso Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Background: In the industrial biotechnology, ligninolytic enzymes are
produced by single fungal strains. Experimental evidence suggests that
co-culture of ligninolytic fungi and filamentous microfungi results in
an increase laccase activity. In this topic, only the ascomycete
Trichoderma spp. has been studied broadly. However, fungal
ligninolytic-filamentousmicrofungi biodiversity interaction in nature
is abundant and poorly studied. The enhancement of laccase and
manganese peroxidase (MnP) activities of Trametes maxima as a
function of time inoculation of Paecilomyces carneus and under
several culture conditions using Plackett-Burman experimental
design (PBED) were investigated. Results: The highest increases of
laccase (12,382.5 U/mg protein) and MnP (564.1 U/mg protein) activities
were seen in co-cultures I3 and I5, respectively, both at 10 d after
inoculation. This level of activity was significantly different from
the enzyme activity in non-inoculated T. maxima (4881.0 U/mg protein
and 291.8 U/mg protein for laccase and MnP, respectively). PBED results
showed that laccase was increased (P < 0.05) by high levels of
glucose, (NH4)2SO4 and MnSO4 and low levels of KH2PO4, FeSO4 and
inoculum (P < 0.05). In addition, MnP activity was increased (P <
0.05) by high yeast extract, MgSO4, CaCl2 and MnSO4 concentrations.
Conclusions: Interaction between indigenous fungi: T. maxima-P.
carneus improves laccase and MnP activities. The inoculation time of P.
carneus on T. maxima plays an important role in the laccase and MnP
enhancement. The nutritional requirements for enzyme improvement in a
co-culture system are different from those required for a monoculture
system. |
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ISSN: | 0717-3458 0717-3458 |
DOI: | 10.1016/j.ejbt.2014.04.007 |