Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and Evaluation of its Protective Effects

• Published in: The Journal of parasitology Vol. 91; no. 2; pp. 476 - 479
• Main Authors: Shirafuji, Hiroaki, Xuan, Xuenan, Kimata, Isao, Takashima, Yasuhiro, Fukumoto, Shinya, Otsuka, Haruki, Nagasawa, Hideyuki, Suzuki, Hiroshi
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01.04.2005; Allen Press Inc
• Subjects: Animals; Antibodies; Antibodies, Protozoan - biosynthesis; Antigens; Biological and medical sciences; Blotting, Western; Calves; Cattle; Cryptosporidiosis; Cryptosporidiosis - prevention & control; Cryptosporidium; Cryptosporidium parvum; Cryptosporidium parvum - genetics; Cryptosporidium parvum - immunology; Disease Models, Animal; E coli; Female; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Gene Expression; General aspects; General aspects and techniques. Study of several systematic groups. Models; Immunization; Immunodominant Epitopes - chemistry; Immunodominant Epitopes - genetics; Immunodominant Epitopes - immunology; Infections; Invertebrates; Liver; Mice; Molecular Weight; Parasites; Parasitology; Proteins; Protozoa; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Protozoan Proteins - immunology; Protozoan Vaccines - biosynthesis; Protozoan Vaccines - genetics; Protozoan Vaccines - immunology; RESEARCH NOTES; Sporozoites; Tachyzoites; Toxoplasma gondii; Vaccination; Vaccines; Vaccines, Synthetic - biosynthesis; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Parasite; Protozoa; Apicomplexa; Toxoplasma gondii; Cryptosporidium parvum
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-364R1

In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse model. The molecular weight and antigenic property of P23 expressed by Tg/P23 were similar to those of the native P23. Mice immunized with lysed Tg/P23 tachyzoites produced specific neutralizing antibodies against C. parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals.