NFIL3 mutations alter immune homeostasis and sensitise for arthritis pathology

• Published in: Annals of the rheumatic diseases Vol. 78; no. 3; pp. 342 - 349
• Main Authors: Schlenner, Susan, Pasciuto, Emanuela, Lagou, Vasiliki, Burton, Oliver, Prezzemolo, Teresa, Junius, Steffie, Roca, Carlos P, Seillet, Cyril, Louis, Cynthia, Dooley, James, Luong, Kylie, Van Nieuwenhove, Erika, Wicks, Ian P, Belz, Gabrielle, Humblet-Baron, Stéphanie, Wouters, Carine, Liston, Adrian
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group LTD 01.03.2019; BMJ Publishing Group
• Subjects: Animals; Arthritis; Arthritis, Juvenile - genetics; Arthritis, Juvenile - immunology; Basic-Leucine Zipper Transcription Factors - genetics; Basic-Leucine Zipper Transcription Factors - immunology; Child; Dendritic cells; Disease Models, Animal; Female; Flow cytometry; Gene expression; Genomes; Homeostasis; Humans; IL-1β; Immune system; Immunity, Innate - genetics; Immunology; Inflammatory bowel disease; Innate immunity; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Lymphocytes; Mice; Mutation; Mutation - immunology; Rheumatoid Arthritis; Transcription factors; Twins, Monozygotic - genetics; Whole Exome Sequencing; Belgium; genetic; macrophages; NFIL3; IL-1β; juvenile idiopathic arthritis
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/annrheumdis-2018-213764

Objectives NFIL3 is a key immunological transcription factor, with knockout mice studies identifying functional roles in multiple immune cell types. Despite the importance of NFIL3, little is known about its function in humans.MethodsHere, we characterised a kindred of two monozygotic twin girls with juvenile idiopathic arthritis at the genetic and immunological level, using whole exome sequencing, single cell sequencing and flow cytometry. Parallel studies were performed in a mouse model.ResultsThe patients inherited a novel p.M170I in NFIL3 from each of the parents. The mutant form of NFIL3 demonstrated reduced stability in vitro. The potential contribution of this mutation to arthritis susceptibility was demonstrated through a preclinical model, where Nfil3-deficient mice upregulated IL-1β production, with more severe arthritis symptoms on disease induction. Single cell sequencing of patient blood quantified the transcriptional dysfunctions present across the peripheral immune system, converging on IL-1β as a pivotal cytokine.ConclusionsNFIL3 mutation can sensitise for arthritis development, in mice and humans, and rewires the innate immune system for IL-1β over-production.