Identification of the Inositol Isomers Present in Tetrahymena

• Published in: The Journal of eukaryotic microbiology Vol. 50; no. 3; pp. 164 - 168
• Main Authors: KERSTING, MICHAEL C, BOYETTE, MICHELE, MASSEY, JOSEPH H, RYALS, PHILLIP E
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.05.2003; Blackwell
• Subjects: Animals; Biochemistry. Physiology. Immunology. Molecular biology; Biological and medical sciences; Cells, Cultured; chiro-Inositol; ciliate; Fundamental and applied biological sciences. Psychology; Glycosylphosphatidylinositols - isolation & purification; Glycosylphosphatidylinositols - metabolism; Inositol - chemistry; Inositol - isolation & purification; Inositol - metabolism; inositol isomer composition of Tetrahymena; Inositol Phosphates - isolation & purification; Inositol Phosphates - metabolism; Isomerism; Lipids - isolation & purification; myo-inositol; neo-inositol; phospholipid; Protozoa; scyllo-inositol; Tetrahymena - chemistry; Tetrahymena vorax; Protozoa; neo-inositol; Inositol; Biochemical analysis; Molecular structure; Phospholipid; scyllo- inositol; Tetrahymena vorax; Isomer; myo-inositol; Tetrahymena; inositol isomer composition of Tetrahymena; ciliate; Ciliata; chiro-Inositol
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/j.1550-7408.2003.tb00111.x

The inositol isomer composition of phosphoinositides, polyphosphoinositols, phosphatidylinositol-linked glycans, and glycosyl phosphatidylinositol-anchored proteins of logarithmic phase Tetrahymena vorax was determined by GC-MS analysis of trimethylsilylimadazole derivatives. The most abundant inositol found was the myo-isomer; however, appreciable percentages of scyllo-inositol were present in the free inositol pool, phosphatidylinositol-linked glycan fraction, and glycosyl phosphatidylinositol-anchored protein fraction. Trace quantities of chiro- and neo-inositols also were present.