Crystallization and preliminary x-ray studies of the Fab fragment from a humanized version of the mouse anti-human fas antibody HFE7a

A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to th...

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Bibliographic Details
Published inProtein and peptide letters Vol. 9; no. 3; p. 259
Main Authors Ito, Shuichiro, Takayama, Tomoko, Hanzawa, Hiroyuki, Takahashi, Tohru, Miyadai, Kenji, Serizawa, Nobufusa, Haruyama, Hideyuki, Hata, Tadashi
Format Journal Article
LanguageEnglish
Published Netherlands 01.06.2002
Subjects
Animals
Antibodies, Monoclonal - chemistry
Crystallography, X-Ray
fas Receptor - chemistry
Humans
Immunoglobulin Fragments - chemistry
Mice
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Summary:A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2(1)2(1)2(1) with cell dimensions a = 54.4 A, b = 82.7 A, c = 104.9 A and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 A resolution.
ISSN:0929-8665
DOI:10.2174/0929866023408715