Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.)

To conduct phylogeographic or population genetic studies, an adequate number of DNA markers for the focal species are required. Due to severe unavailability of genotype markers of any kind for the species Eurasian minnow (Phoxinus phoxinus L.) and rudd (Scardinius erythrophthalmus L.), we set out to...

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Published inBMC research notes Vol. 2; no. 1; p. 248
Main Authors Holmen, Johannes, Vøllestad, Leif A, Jakobsen, Kjetill S, Primmer, Craig R
Format Journal Article
LanguageEnglish
Published England BioMed Central 14.12.2009
BioMed Central Ltd
BMC
Subjects
Annealing
Genetics
Short Report
Studies
Zebrafish
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Summary:To conduct phylogeographic or population genetic studies, an adequate number of DNA markers for the focal species are required. Due to severe unavailability of genotype markers of any kind for the species Eurasian minnow (Phoxinus phoxinus L.) and rudd (Scardinius erythrophthalmus L.), we set out to attempt cross-amplification of a set of microsatellite loci from related species. We tested 36 cyprinid microsatellite loci for cross-species amplification in minnow and rudd. Fifteen species-locus combinations produced amplifications in minnow, seven being polymorphic, while 18 combinations amplified in rudd, nine of these being polymorphic. The positive cross-species amplifications present potential contributions to the establishment of genetic marker sets for population genetics studies of the two focal species.
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ISSN:1756-0500
1756-0500
DOI:10.1186/1756-0500-2-248