Evidence for the presence of hepatitis E virus in pigs in the United Kingdom
Samples of serum, tissue and faeces from two pig herds in England were examined for hepatitis E virus by reverse-transcriptase PCR (RT-PcR), and a virus strain from each herd was partially sequenced. Eleven of 42 faecal samples and 16 of 21 tissue samples from two pigs were positive for the virus by...
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Published in | Veterinary record Vol. 154; no. 8; pp. 223 - 227 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
BMJ Publishing Group Limited
21.02.2004
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Samples of serum, tissue and faeces from two pig herds in England were examined for hepatitis E virus by reverse-transcriptase PCR (RT-PcR), and a virus strain from each herd was partially sequenced. Eleven of 42 faecal samples and 16 of 21 tissue samples from two pigs were positive for the virus by RT-PCR. Analysis of two unique but closely related nucleotide sequences obtained from the two herds showed that the viruses clustered in genotype III (6) with a human strain of the virus from an autochthonously acquired case of acute hepatitis in the uK. An ELISA based on recombinant open reading frame 2 (ORF-2) was used to detect antibodies to hepatitis E virus in 256 pig sera from the uK; 85-5 per cent of the samples were positive, compared with 58 per cent of similar samples from Swedish pigs and 23-5 per cent of samples from Dutch pigs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0042-4900 2042-7670 |
DOI: | 10.1136/vr.154.8.223 |