Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 103; no. 4; pp. 386 - 391
• Main Authors: Gouveia, Ana Cláudia Carvalho, Eisenach, Kathleen D, Vinhas, Solange Alves, Ribeiro, Fabiola Karla Corrêa, Peres, Renata Lyrio, Dietze, Reynaldo, Hadad, David Jamil, Palaci, Moisés
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01.06.2008; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Algorithms; BACTEC® 12B; BACTEC® 12B - culture; culture; Culture Media; diagnosis; DNA, Bacterial - analysis; Humans; Hydroxypropiophenone - analogs & derivatives; identification; Mycobacterium tuberculosis; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - isolation & purification; PARASITOLOGY; PCR; Phenotype; Polymerase Chain Reaction; Sensitivity and Specificity; Sputum - microbiology; Time Factors; TROPICAL MEDICINE; Tuberculosis, Pulmonary - diagnosis; Tuberculosis, Pulmonary - microbiology; diagnosis; identification; Mycobacterium tuberculosis; BACTEC® 12B; culture; PCR

We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC® 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC® 12B vials. At a growth index (GI) > 30, 1.0 ml of the 12B broth was rem...