Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens
We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC® 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC® 12B vials. At a growth index (GI) > 30, 1.0 ml of the 12B broth was rem...
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Published in | Memórias do Instituto Oswaldo Cruz Vol. 103; no. 4; pp. 386 - 391 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English Portuguese |
Published |
Brazil
Fundação Oswaldo Cruz, Fiocruz
01.06.2008
Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ) |
Subjects | |
Online Access | Get full text |
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Summary: | We evaluated the ability of a PCR assay to identify Mycobacterium
tuberculosis complex (MTBC) from positive BACTEC® 12B broth
cultures. A total of 107 sputum samples were processed and inoculated
into Ogawa slants and BACTEC® 12B vials. At a growth index (GI)
> 30, 1.0 ml of the 12B broth was removed, stored, and assayed with
PCR. Molecular results were compared to those obtained by phenotypic
identification methods, including the BACTEC® NAP method. The
average times required to perform PCR and NAP were compared. Of the 107
broth cultures evaluated, 90 were NAP positive, while 91 were PCR
positive for MTBC. Of particular interest were three contaminated
BACTEC® 12B broth cultures yielding microorganisms other than
acid-fast bacilli growth with a MTBC that were successfully identified
by PCR, resulting in a mean time of 14 days to identify MTBC before NAP
identification. These results suggest that PCR could be used as an
alternative to the NAP test for the rapid identification of MTBC in
BACTEC® 12B cultures, particularly in those that contained both
MTBC and nontuberculous mycobacteria. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1678-8060 0074-0276 1678-8060 0074-0276 |
DOI: | 10.1590/S0074-02762008000400012 |