Immunofluorescence detection of new antigen-antibody system (delta/anti-delta) associated to hepatitis B virus in liver and in serum of HBsAg carriers

• Published in: Gut Vol. 18; no. 12; pp. 997 - 1003
• Main Authors: Rizzetto, M, Canese, M G, Aricò, S, Crivelli, O, Trepo, C, Bonino, F, Verme, G
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and British Society of Gastroenterology 01.12.1977; BMJ Publishing Group LTD
• Subjects: Adult; Antibodies, Viral - analysis; Antigen-Antibody Reactions; Cell Nucleus - immunology; Chronic Disease; Epitopes; Female; Fluorescent Antibody Technique; Hepatitis B; Hepatitis B Antibodies - analysis; Hepatitis B Antigens - analysis; Hepatitis B Surface Antigens; Humans; Liver - immunology; Liver - ultrastructure; Liver Diseases - immunology; Male; Microscopy, Electron; Middle Aged
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.18.12.997

A new antigen-antibody system associated with the hepatitis B virus and immunologically distinct from the HB surface, core, and e systems is reported. The new antigen, termed delta, was detected by direct immunofluorescence only in the liver cell nuclei of patients with HBsAg positive chronic liver disease. At present, the intrahepatic expression of HBcAg and delta antigen appears to be mutually exclusive. No ultrastructural aspect corresponding to the delta antigen could be identified under the electron microscope. delta antibody was found in the serum of chronic HBsAg carriers, with a higher prevalence in patients with liver damage. The nuclear fluorescence patterns of HBcAg and delta antigen were similar; it is only possible to discriminate between the two antigens by using the respective specific antisera.