Mini-review: recent developments in hydroxynitrile lyases for industrial biotechnology

Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a broad range of c...

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Bibliographic Details
Published inRecent patents on biotechnology Vol. 7; no. 3; p. 197
Main Authors Lanfranchi, Elisa, Steiner, Kerstin, Glieder, Anton, Hajnal, Ivan, Sheldon, Roger A, van Pelt, Sander, Winkler, Margit
Format Journal Article
LanguageEnglish
Published United Arab Emirates 01.12.2013
Subjects
Aldehyde-Lyases - genetics
Aldehyde-Lyases - metabolism
Biotechnology
Catalysis
Nitriles - metabolism
Patents as Topic
Stereoisomerism
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Summary:Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a broad range of chemical and enzymatic follow-up reactions. A significant number of (R)- and (S)-selective HNLs are known today and the number is still increasing. HNLs not only exhibit varying substrate scope but also differ in sequence and structure. Tailor-made enzymes for large-scale manufacturing of cyanohydrins with improved yield and enantiomeric excess are very interesting targets, which is reflected in a solid number of patents. This review will complement and extend our recent review with a strong focus on applications of HNLs for the synthesis of highly functionalized, chiral compounds with newest literature, recent and current patent literature.
ISSN:2212-4012
DOI:10.2174/18722083113076660010