Human pulmonary macrophages: the relationship of smoking to the presence of sea blue granules and surfactant turnover

• Published in: Journal of clinical pathology Vol. 33; no. 8; pp. 738 - 743
• Main Authors: Plowman, P N, Flemans, R J
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.08.1980; BMJ Publishing Group LTD
• Subjects: Adult; Cytoplasmic Granules - ultrastructure; Humans; Lung - metabolism; Lung - ultrastructure; Macrophages - metabolism; Macrophages - ultrastructure; Male; Microscopy, Electron; Pulmonary Alveoli - ultrastructure; Pulmonary Surfactants - metabolism; Staining and Labeling; Tobacco Use Disorder - metabolism; Tobacco Use Disorder - pathology
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.33.8.738

Human pulmonary macrophages from heavy smokers usually (17/21 cases) contain sea blue inclusion material in the cytoplasm. In non-smokers, sea blue material is rarely evident (1/21 cases). The sea blue material observed has the same properties as sea blue material identified in other conditions (viz, Sudan black and PAS positivity and autofluorescence). Evidence is presented which suggests that pulmonary macrophages from smokers contain extra surfactant and that it is this phospholipid complex that accounts for the sea blue reaction observed.