Conception and Realization of a Non-Cationic Non-Viral DNA Vector

Cationic non-viral DNA vectors are very successful in in vitro transfections but less efficient in in vivo tests. This seems mainly due to the cationic nature of the molecules used to complex DNA. In this article, we describe the design and the route towards the realization of a non-viral non-cation...

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Bibliographic Details
Published inCurrent medicinal chemistry Vol. 11; no. 2; pp. 169 - 177
Main Authors Roux, D, Chenevier, P, Pott, T, Navailles, L, Regev, O, Mondain Monval, O
Format Journal Article
LanguageEnglish
Published United Arab Emirates Bentham Science Publishers Ltd 01.01.2004
Subjects
Animals
Cations
Deoxyribonucleic acid
DNA
DNA, Viral
Genetic Vectors - chemistry
Genetic Vectors - genetics
Humans
Liposomes - chemistry
Peptides
Phosphatidylcholines - chemistry
Water - chemistry
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Summary:Cationic non-viral DNA vectors are very successful in in vitro transfections but less efficient in in vivo tests. This seems mainly due to the cationic nature of the molecules used to complex DNA. In this article, we describe the design and the route towards the realization of a non-viral non-cationic vector. The strategy follows three steps: first, the incorporation of DNA to a lamellar phase; second, the making of multilamellar vesicles containing a high loading of DNA by shearing the lamellar phase and, finally, the grafting of peptides onto the surface of the vesicles to target a specific receptor on the cells. Throughout this process, we had to overcome many obstacles; this review describes the present state of our work and summarizes the remaining steps.
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ISSN:0929-8673
1875-533X
DOI:10.2174/0929867043456133