Pharmaco-Phylogenetic Investigation of Methyl Gallate Isolated from Acacia nilotica (L.) Delile and Its Cytotoxic Effect on NIH3T3 Mouse Fibroblast

• Published in: Current pharmaceutical biotechnology Vol. 17; no. 6; p. 540
• Main Authors: Mishra, Rohit K, Ramakrishna, M, Mishra, Vani, Pathak, Ashutosh, Rajesh, S, Sharma, Shivesh, Pandey, Avinash C, Nageswara Rao, G, Dikshit, Anupam
• Format: Journal Article
• Language: English
• Published: Netherlands 01.05.2016
• Subjects: Acacia; Animals; Anti-Bacterial Agents - pharmacology; Bacteria - drug effects; Bacteria - growth & development; Cell Survival - drug effects; Gallic Acid - analogs & derivatives; Gallic Acid - pharmacology; Mice; Microbial Sensitivity Tests; NIH 3T3 Cells; Phylogeny; Plant Extracts; Plant Leaves
• ISSN: 1873-4316
• DOI: 10.2174/1389201017666160127110759

Present exploration deals with the therapeutic perspective of methyl gallate isolated from the leaf extract of Acacia nilotica (L.) Delile in contrast to food-borne bacterial pathogen's viz., Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus aureus with their evolutionary succession. The extract was subjected to phytochemical analysis and isolated compound was identified as methyl gallate using UV-vis, IR and NMR spectra. It was found most potent against K. pneumoniae with its minimum inhibition concentration (MIC) of 0.32 mg/ml and minimum bactericidal concentration (MBC) at 0.62 mg/ml. The correlation of MIC values with an evolutionary succession assists the relationship between their genetic and toxic properties. The cytotoxic pursuit of methyl gallate was additionally assessed over NIH3T3 mouse fibroblast by Neutral red (NR) uptake, MTT cell proliferation assay and did not disclose any relevant influence on cell viability as well as cell proliferation. As such, the methyl gallate extracted from the leaf of A. nilotica holds massive antibacterial aptitude and hands out towards a new paradigm for food and pharmaceutical industries.