Deviated and early unsustainable stunted development of gut microbiota in children with autism spectrum disorder

• Published in: Gut Vol. 71; no. 8; pp. 1588 - 1599
• Main Authors: Lou, Mingxing, Cao, Aihua, Jin, Cuiyuan, Mi, Kai, Xiong, Xiyue, Zeng, Zhaoyang, Pan, Xu, Qie, Jinlong, Qiu, Shangfeng, Niu, Yaofang, Liang, Hao, Liu, Yanping, Chen, Lin, Liu, Zhi, Zhao, Qing, Qiu, Xiyan, Jin, Yuanxiang, Sheng, Xiaoqi, Hu, Zhibin, Jin, Gulei, Liu, Jingshi, Liu, Xingyin, Wang, Yichao
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and British Society of Gastroenterology 01.08.2022; BMJ Publishing Group LTD; BMJ Publishing Group
• Series: Original research
• Subjects: Age; Autism; Autism spectrum disorder; Autism Spectrum Disorder - metabolism; Autistic children; Birth defects; Child; Children; Childrens health; Cohort Studies; Gastrointestinal Microbiome - genetics; Gut Microbiota; Humans; Intestinal microflora; Laboratories; Maternal & child health; Microbiomes; Microbiota; Neurotypical; Provinces; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sample size; China; Gut microbiota; Neurotypical; Autism spectrum disorder
• ISSN: 0017-5749; 1468-3288; 1468-3288
• DOI: 10.1136/gutjnl-2021-325115

ObjectiveRecent studies have provided insights into the gut microbiota in autism spectrum disorder (ASD); however, these studies were restricted owing to limited sampling at the unitary stage of childhood. Herein, we aimed to reveal developmental characteristics of gut microbiota in a large cohort of subjects with ASD combined with interindividual factors impacting gut microbiota.DesignA large cohort of 773 subjects with ASD (aged 16 months to 19 years), 429 neurotypical (NT) development subjects (aged 11 months to 15 years) were emolyed to determine the dynamics change of gut microbiota across different ages using 16S rRNA sequencing.ResultIn subjects with ASD, we observed a distinct but progressive deviation in the development of gut microbiota characterised by persistently decreased alpha diversity, early unsustainable immature microbiota, altered aboudance of 20 operational taxonomic units (OTUs), decreased taxon detection rate and 325 deregulated microbial metabolic functions with age-dependent patterns. We further revealed microbial relationships that have changed extensively in ASD before 3 years of age, which were associated with the severity of behaviour, sleep and GI symptoms in the ASD group. This analysis demonstrated that a signature of the combination of 2 OTUs, Veillonella and Enterobacteriaceae, and 17 microbial metabolic functions efficiently discriminated ASD from NT subjects in both the discovery (area under the curve (AUC)=0.86), and validation 1 (AUC=0.78), 2 (AUC=0.82) and 3 (AUC=0.67) sets.ConclusionOur large cohort combined with clinical symptom analysis highlights the key regulator of gut microbiota in the pathogenesis of ASD and emphasises the importance of monitoring and targeting the gut microbiome in future clinical applications of ASD.