Dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus

• Published in: Gut Vol. 67; no. 9; pp. 1614 - 1625
• Main Authors: Wang, Jinfeng, Zheng, Jiayong, Shi, Wenyu, Du, Nan, Xu, Xiaomin, Zhang, Yanming, Ji, Peifeng, Zhang, Fengyi, Jia, Zhen, Wang, Yeping, Zheng, Zhi, Zhang, Hongping, Zhao, Fangqing
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group LTD 01.09.2018; BMJ Publishing Group
• Series: Original article
• Subjects: Adult; Amniotic fluid; Bacteria; Bioinformatics; Birth; Blood Glucose - metabolism; Cesarean Section - methods; Deoxyribonucleic acid; Diabetes; Diabetes mellitus; Diabetes, Gestational - blood; Diabetes, Gestational - diagnosis; Diabetes, Gestational - metabolism; Disease; DNA; Dysbacteriosis; Dysbiosis - complications; Dysbiosis - metabolism; Female; Genomes; Glucose tolerance; Glucose Tolerance Test - methods; Gut Microbiota; Humans; Infant, Newborn; Intestine; Meconium; Metabolism; Microbiomes; Microbiota; Neonates; Pediatrics; Pharynx; Pregnancy; Premature birth; Vagina; Womens health; Beijing China; China; microbiota; GDM; neonate; pregnancy
• ISSN: 0017-5749; 1468-3288
• DOI: 10.1136/gutjnl-2018-315988

ObjectiveThe initial colonisation of the human microbiota and the impact of maternal health on neonatal microbiota at birth remain largely unknown. The aim of our study is to investigate the possible dysbiosis of maternal and neonatal microbiota associated with gestational diabetes mellitus (GDM) and to estimate the potential risks of the microbial shift to neonates.DesignPregnant women and neonates suffering from GDM were enrolled and 581 maternal (oral, intestinal and vaginal) and 248 neonatal (oral, pharyngeal, meconium and amniotic fluid) samples were collected. To avoid vaginal bacteria contaminations, the included neonates were predominantly delivered by C-section, with their samples collected within seconds of delivery.ResultsNumerous and diverse bacterial taxa were identified from the neonatal samples, and the samples from different neonatal body sites were grouped into distinct clusters. The microbiota of pregnant women and neonates was remarkably altered in GDM, with a strong correlation between certain discriminatory bacteria and the oral glucose tolerance test. Microbes varying by the same trend across the maternal and neonatal microbiota were observed, revealing the intergenerational concordance of microbial variation associated with GDM. Furthermore, lower evenness but more depletion of KEGG orthologues and higher abundance of some viruses (eg, herpesvirus and mastadenovirus) were observed in the meconium microbiota of neonates associated with GDM.ConclusionGDM can alter the microbiota of both pregnant women and neonates at birth, which sheds light on another form of inheritance and highlights the importance of understanding the formation of early-life microbiome.