In Vitro Antibacterial Activity of a 7-O- β -D-glucopyranosyl-nutanocoumarin from Chaptalia nutans (Asteraceae)

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 98; no. 2; pp. 283 - 286
• Main Authors: Truiti, Maria da Conceião Torrado, Sarragiotto, Maria Helena, de Abreu Filho, Benício Alves, Nakamura, Celso Vataru, Dias Filho, Benedito Prado
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01.03.2003; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: 7-O- β -D-glucopyranosyl-nutanocoumarin; 7-O-beta-D-glucopyranosyl-nutanocoumarin; Anti-Bacterial Agents - pharmacology; antibacterial activity; Asteraceae; Chaptalia nutans; Coumarins - pharmacology; Escherichia coli - drug effects; Glucosides - pharmacology; Microbial Sensitivity Tests; PARASITOLOGY; Plant Extracts - pharmacology; Plants, Medicinal; Pseudomonas aeruginosa - drug effects; Staphylococcus aureus - drug effects; traditional medicine; TROPICAL MEDICINE; antibacterial activity; Chaptalia nutans; 7-O-beta-D-glucopyranosyl-nutanocoumarin; Asteraceae; traditional medicine
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762003000200020

Ethanolic crude extracts from the roots of Chaptalia nutans , traditionally used in Brazilian folk medicine, were screened against Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa by using the disk diffusion test technique. S. aureus with 14 mm inhibition zone was considered susceptible. E. coli and P. aeruginosa without such a zone were considered resistant. As a result of this finding, the ethanolic crude extract was fractionated on silica gel column chromatography into five fractions. The ethyl acetate fraction was active against S. aureus and Bacillus subtilis . Further column chromatography separation of the ethyl acetate fraction afforded 30 fractions, which were assayed against S. aureus . Fractions 16 and 17 showed inhibition zones with S. aureus , indicating the presence of active compounds, and were subjected to purification by repeated preparative thin layer chromatography. The pure compound 7-O- β -D-glucopyranosyl-nutanocoumarin inhibited B. subtilis and S. aureus at concentrations of 62.5 µ g/ml and 125 µ g/ml, respectively. The antibacterial property of C. nutans appears to have justified its use for the treatment of wounds, which are contaminated through bacterial infections.