Internal birefringence and the recognition of Leishmania parasites

The authors have recently noted, in the bone marrow of a patient found to have kala azar, that the kinetoplast of the amastigote form of Leishmania donovani is sharply birefringent under polarized light. Study of several specimens of different Leishmania species in Romanowsky-stained smears (one of...

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Published inJournal of clinical pathology Vol. 36; no. 5; pp. 604 - 605
Main Authors Flemans, R J, Hayhoe, F G
Format Journal Article
LanguageEnglish
Published England BMJ Publishing Group Ltd and Association of Clinical Pathologists 01.05.1983
BMJ Publishing Group LTD
Subjects
Animals
Birefringence
Bone Marrow - parasitology
Humans
Leishmania
Leishmania - isolation & purification
Leishmaniasis, Visceral - diagnosis
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Summary:The authors have recently noted, in the bone marrow of a patient found to have kala azar, that the kinetoplast of the amastigote form of Leishmania donovani is sharply birefringent under polarized light. Study of several specimens of different Leishmania species in Romanowsky-stained smears (one of L. donovani (man) and others of hamster tissues infected respectively with L. tropica major (USSR, rodent), L. braziliensis braziliensis (Brazil, man), L. donovani infantum (Ethiopia, man) and L. mexicana amazonensis (Brazil, rodent) has confirmed this observation as valid for all parasites studied.
Bibliography:PMID:6841654
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ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.36.5.604