Microsatellite Markers for the New Zealand Endemic Myosotis pygmaea Species Group (Boraginaceae) Amplify Across Species

Premise of the study: Microsatellite loci were developed as polymorphic markers for the New Zealand endemic Myosotis pygmaea species group (Boraginaceae) for use in species delimitation and population and conservation genetic studies. Methods and Results: Illumina MiSeq sequencing was performed on g...

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Published inApplications in plant sciences Vol. 3; no. 6
Main Authors Prebble, Jessica M, Tate, Jennifer A, Meudt, Heidi M, Symonds, V. Vaughan
Format Journal Article
LanguageEnglish
Published United States Botanical Society of America 01.06.2015
John Wiley & Sons, Inc
Subjects
Boraginaceae
Conserved sequence
Deoxyribonucleic acid
DNA
DNA sequencing
Endangered & extinct species
Endemic species
forget-me-nots
Genetic diversity
Genetic markers
Genomes
microsatellite repeats
Microsatellites
Myosotis
National parks
New Zealand
Nucleotide sequence
Plant sciences
population
Population genetics
Population studies
PRIMER NOTES
Seedlings
threatened species
New Zealand
South Island New Zealand
North Island
California
United States > US
Myosotis
New Zealand
microsatellites
forget-me-nots
Boraginaceae
threatened species
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Summary:Premise of the study: Microsatellite loci were developed as polymorphic markers for the New Zealand endemic Myosotis pygmaea species group (Boraginaceae) for use in species delimitation and population and conservation genetic studies. Methods and Results: Illumina MiSeq sequencing was performed on genomic DNA from seedlings of M. drucei. From trimmed paired-end sequences >400 bp, 484 microsatellite loci were identified. Twelve of 48 microsatellite loci tested were found to be polymorphic and consistently scorable when screened on 53 individuals from four populations representing the geographic range of M. drucei. They also amplify in all other species in the M. pygmaea species group, i.e., M. antarctica, M. brevis, M. glauca, and M. pygmaea, as well as 18 other Myosotis species. Conclusions: These 12 polymorphic microsatellite markers establish an important resource for research and conservation of the M. pygmaea species group and potentially other Southern Hemisphere Myosotis.
Bibliography:http://dx.doi.org/10.3732%2Fapps.1500027
The authors thank Te Papa and Massey University for funding, including a Massey University Vice‐Chancellor's Doctoral Scholarship to J.M.P. Fieldwork was facilitated by the Australasian Systematic Botany Society Eichler Award, the Royal Society of New Zealand's Hutton Fund, and the New Zealand Department of Conservation (permit number CA‐31615‐OTH). This research was supported by core funding for Crown Research Institutes from the Ministry of Business, Innovation and Employment's Science and Innovation Group.
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ISSN:2168-0450
2168-0450
DOI:10.3732/apps.1500027