Dynamics in the Expression of Epigenetic Modifiers and Histone Modifications in Perinatal Rat Germ Cells during De Novo DNA Methylation

• Published in: Biology of reproduction Vol. 104; no. 2; pp. 361 - 373
• Main Authors: Rwigemera, Arlette, Omri-Charai, Rhizlane El, Lecante, Laetitia L., Delbes, Geraldine
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 01.02.2021; Oxford University Press
• Subjects: Acetates; Chromatin; DNA; DNA methylation; Enzymes; Epigenetic inheritance; epigenetic modifiers; Epigenetics; Females; Fluorescence; Genetic engineering; Genetic transcription; Genomes; gonocytes; histone posttranslational modifications; Males; Methylation; Methyltransferases; perinatal life; rat; RESEARCH ARTICLE; Rodents; Scientific equipment and supplies industry; United States; Quebec; histone posttranslational modifications; DNA methylation; perinatal life; rat; epigenetic modifiers; gonocytes
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/ioaa206

Epigenetic reprogramming during perinatal germ cell development is essential for genomic imprinting and cell differentiation; however, the actors of this key event and their dynamics are poorly understood in rats. Our study aimed to characterize the expression patterns of epigenetic modifiers and the changes in histone modifications in rat gonocytes at the time of de novo DNA methylation. Using transgenic rats expressing Green Fluorescent Protein (GFP) specifically in germ cells, we purified male gonocytes by fluorescent activated cell sorting at various stages of perinatal development and established the transcriptomic profile of 165 epigenetic regulators. Using immunofluorescence on gonad sections, we tracked six histone modifications in rat male and female perinatal germ cells over time, including methylation of histone H3 on lysines 27, 9, and 4; ubiquitination of histone H2A on lysine119; and acetylation of histone H2B on lysine 20. The results revealed the dynamics in the expression of ten-eleven translocation enzymes and DNA methyltransferases in male gonocytes at the time of de novo DNA methylation. Moreover, our transcriptomic data indicate a decrease in histone ubiquitination and methylation coinciding with the beginning of de novo DNA methylation. Decreases in H2AK119Ub and H3K27me3 were further confirmed by immunofluorescence in the male germ cells but were not consistent for all H3 methylation sites examined. Together, our data highlighted transient chromatin remodeling involving histone modifications during de novo DNA methylation. Further studies addressing how these dynamic changes in histone posttranslational modifications could guide de novo DNA methylation will help explain the complex establishment of the male germ cell epigenome. Summary sentence De novo DNA methylation in rat gonocytes is associated with a transient modulation of the transcription of epigenetic modifiers involved in DNA methylation and histone posttranslational modifications, indicating a dynamic chromatin remodeling. Graphical Abstract