Detection of Toxoplasma gondii by polymerase chain reaction in sera of acutely infected mice

• Published in: The Journal of parasitology Vol. 86; no. 4; p. 857
• Main Authors: Hafid, J, Guichard, D, Flori, P, Bourlet, T, Raberin, H, Genin, C, Sung, R T
• Format: Journal Article
• Language: English
• Published: United States 01.08.2000
• Subjects: Acute Disease; Animals; DNA, Protozoan - blood; Electrophoresis, Agar Gel; Female; Mice; Parasitemia - diagnosis; Parasitemia - parasitology; Polymerase Chain Reaction; Sensitivity and Specificity; Toxoplasma - genetics; Toxoplasma - isolation & purification; Toxoplasmosis, Animal - diagnosis; Toxoplasmosis, Animal - parasitology
• ISSN: 0022-3395
• DOI: 10.1645/0022-3395(2000)086[0857:DOTGBP]2.0.CO;2

The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 x 10(3) T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.