Effects of space flight on the immunohistochemical demonstration of connexin 26 and connexin 43 in the postpartum uterus of rats

• Published in: Journal of reproduction & fertility Vol. 116; no. 2; pp. 229 - 234
• Main Authors: Burden, H W, Zary, J, Alberts, J R
• Format: Journal Article
• Language: English
• Published: England Society for Reproduction and Fertility 01.07.1999
• Subjects: Analysis of Variance; Animals; Connexin 26; Connexin 43 - analysis; Connexins - analysis; Endometrium - chemistry; Female; Gestational Age; Immunohistochemistry; Labor, Obstetric - metabolism; Myometrium - chemistry; Postpartum Period - metabolism; Pregnancy; Rats; Rats, Wistar; Space Flight; Uterine Contraction; Uterus - chemistry; NASA Discipline Developmental Biology; NASA Experiment Number 9303031 2/2; Non-NASA Center
• ISSN: 1470-1626; 0022-4251; 1741-7899
• DOI: 10.1530/jrf.0.1160229

The effect of space flight in a National Aeronautics and Space Administration shuttle was studied in pregnant rats. Rats were launched on day 11 of gestation and recovered on day 20 of gestation. Pregnancy was allowed to proceed to term and rats delivered vaginally on days 22–23, although flight animals required more labour contractions to complete the delivery process. Pups were placed with foster dams and connexin 26 and 43 were examined in the uterus of flight animals approximately 3 h after delivery. Space flight did not affect uterine connexin 26, localized primarily in epithelial cells of the endometrium, but decreased connexin 43, the major gap junction protein in the myometrium. It is suggested that decreased connexin 43 alters synchronization and coordination of labour contractions, resulting in a requirement for more contractions to complete the delivery process.