Immunocytochemical demonstration of oxytocin in bovine ovarian tissues

The presence of oxytocin in ovarian tissue was examined immunocytochemically. Bovine antral follicles and corpora lutea were fixed with glutaraldehyde, picric acid and acetic acid fixative and immuno-stained by the indirect peroxidase-antiperoxydase (PAP) technique. Immunoreactive oxytocin was demon...

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Published inActa endocrinologica (Copenhagen) Vol. 109; no. 4; pp. 537 - 542
Main Authors Kruip, T A, Vullings, H G, Schams, D, Jonis, J, Klarenbeek, A
Format Journal Article
LanguageEnglish
Published Denmark 01.08.1985
Subjects
Animals
Cattle
Corpus Luteum - cytology
Corpus Luteum - metabolism
Female
Follicular Phase
Immunoenzyme Techniques
Luteal Phase
Ovarian Follicle - cytology
Ovarian Follicle - metabolism
Ovaries
Oxytocin - analysis
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Summary:The presence of oxytocin in ovarian tissue was examined immunocytochemically. Bovine antral follicles and corpora lutea were fixed with glutaraldehyde, picric acid and acetic acid fixative and immuno-stained by the indirect peroxidase-antiperoxydase (PAP) technique. Immunoreactive oxytocin was demonstrated in the granulosa cells of small and large follicles, in the granulosalutein cells of the young corpus luteum and in the large luteal cells of the mature corpus luteum. The regressing corpus luteum was not stainable. It is discussed that these findings additionally support the view that oxytocin is actually synthesized in ovarian tissues.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.1090537