Microsatellite Markers for the Invasive Species Bidens alba (Asteraceae)

• Published in: Applications in plant sciences Vol. 2; no. 5; pp. 1400008 - n/a
• Main Authors: Lu, Yong-Bin, Huang, Dong-Ling, Wang, Xie, Wu, Zheng-Jun, Tang, Shao-Qing
• Format: Journal Article
• Language: English
• Published: United States Botanical Society of America 01.05.2014; John Wiley & Sons, Inc; Wiley
• Subjects: alleles; Asteraceae; Bidens; Bidens alba; Deoxyribonucleic acid; DNA; DNA primers; Genetic diversity; Genetic markers; Genetic structure; genetic variation; Heterozygosity; Introduced species; Invasive species; loci; microsatellite marker; microsatellite repeats; Microsatellites; Plant sciences; Population structure; Primer Notes; sequence analysis; simple sequence repeat (SSR); China; Central America; Palo Alto California; California; United States > US; Connecticut; simple sequence repeat (SSR); microsatellite marker; Asteraceae; Bidens alba
• ISSN: 2168-0450; 2168-0450
• DOI: 10.3732/apps.1400008

Premise of the study: Microsatellite markers were developed in the invasive species Bidens alba (Asteraceae) to assess its population structure and to facilitate tracking its expansion in China. Methods and Results: Using 454 pyrosequencing, 20 microsatellite primer sets were developed for B. alba. The markers were tested on one population of B. alba (30 individuals) and one population of the closely related B. pilosa (30 individuals) in China. For B. alba, all of the markers were polymorphic, and the number of alleles per locus ranged from three to 32. The expected heterozygosity values were from 0.3787 to 0.9284, and the Shannon–Wiener index was from 0.6796 to 2.8401. Conclusions: These markers will be useful for investigating the genetic structure, genetic diversity, and invasion dynamics of B. alba and will also be useful in studies of B. pilosa.