Expression of capacitation-dependent changes in chlortetracycline fluorescence patterns in mouse spermatozoa requires a suitable glycolysable substrate
Chlortetracycline (CTC) fluorescence patterns were assessed in epididymal mouse sperm suspensions capacitated in exogenous substrate-containing and substrate-free media. A capacitation-dependent transition from a majority of acrosome-intact cells expressing the uncapacitated F pattern of fluorescenc...
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Published in | Journal of reproduction & fertility Vol. 88; no. 2; pp. 611 - 621 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Society for Reproduction and Fertility
01.03.1990
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Subjects | |
Online Access | Get full text |
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Summary: | Chlortetracycline (CTC) fluorescence patterns were assessed in epididymal mouse sperm suspensions capacitated in exogenous
substrate-containing and substrate-free media. A capacitation-dependent transition from a majority of acrosome-intact cells
expressing the uncapacitated F pattern of fluorescence to a majority with the capacitated acrosome-intact B and acrosome-reacted
AR patterns was confirmed for suspensions incubated a total of 120 min in the presence of a glycolysable substrate, glucose.
In contrast, assessment of spermatozoa incubated for 120 min in substrate-free medium revealed a majority of cells with the
uncapacitated F pattern, despite an earlier demonstration that such cells are essentially capacitated: upon the introduction
of glucose, suspensions are immediately highly fertile. When a suitable glycolysable substrate, either glucose or mannose
but not fructose, was added to such suspensions, the distribution of CTC patterns changed within 10 min to a majority of B
and AR patterns. Furthermore, the degree of change from uncapacitated to capacitated patterns was substrate concentration-dependent.
In contrast, the introduction of the non-metabolizable substrates 2-deoxyglucose and 3-0-methylglucose and the oxidizable
substrates sodium pyruvate and sodium lactate caused no change in the patterns from those seen in substrate-free medium. The
in-vitro fertilizing ability of sperm suspensions to which increasing amounts of glucose or mannose were added, after initial
substrate-free preincubation, directly paralleled the changes in CTC patterns and was as rapid as for suspensions incubated
continuously in either hexose. We therefore conclude that the alteration in position of surface components to which CTC binds
is not only capacitation-dependent, but also energy-dependent. In the absence of an appropriate exogenous glycolysable substrate,
the final transition cannot occur, even though the cells are essentially capacitated.
Keywords: chlortetracycline; capacitation; glycolysis; acrosome reaction; mouse |
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ISSN: | 1470-1626 0022-4251 1741-7899 |
DOI: | 10.1530/jrf.0.0880611 |