Decoding the transcriptome of pre-granulosa cells during the formation of primordial follicles in the mouse

Primordial follicles, a finite reservoir of eggs in mammalian ovaries, are composed of a single oocyte and its supporting somatic cells, termed granulosa cells. Although their formation may require reciprocal interplay between oocytes and pre-granulosa cells, precursors of granulosa cells, little is...

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Published inBiology of reproduction Vol. 105; no. 1; pp. 179 - 191
Main Authors Fukuda, Kurumi, Muraoka, Masafumi, Kato, Yuzuru, Saga, Yumiko
Format Journal Article
LanguageEnglish
Published United States Society for the Study of Reproduction 01.07.2021
Oxford University Press
Subjects
Animals
Cell adhesion
Cell differentiation
Extracellular matrix
Female
Follicles
Gene expression
Gene Expression Regulation, Developmental
Germ Cells - metabolism
granulosa cell
Granulosa cells
Granulosa Cells - metabolism
Mice
Mutants
Oocytes
Oocytes - metabolism
oogenesis
Ovarian Follicle - growth & development
Ovaries
primordial follicle formation
RESEARCH ARTICLE
Signal Transduction
Somatic cells
Transcriptome
Transcriptomes
Japan
primordial follicle formation
granulosa cell
transcriptome
oogenesis
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Summary:Primordial follicles, a finite reservoir of eggs in mammalian ovaries, are composed of a single oocyte and its supporting somatic cells, termed granulosa cells. Although their formation may require reciprocal interplay between oocytes and pre-granulosa cells, precursors of granulosa cells, little is known about the underlying mechanisms. We addressed this issue by decoding the transcriptome of pre-granulosa cells during the formation of primordial follicles. We found that marked gene expression changes, including extracellular matrix, cell adhesion, and several signaling pathways, occur along with primordial follicle formation. Importantly, differentiation of Lgr5-EGFP-positive pre-granulosa cells to FOXL2-positive granulosa cells was delayed in mutant ovaries of the germ cell-specific genes Nanos3 and Figla, accompanied by perturbed gene expression in mutant pre-granulosa cells. These results suggest that proper development of oocytes is required for the differentiation of pre-granulosa cells. Our data provide a valuable resource for understanding the gene regulatory networks involved in the formation of primordial follicles. Summary sentence Gene expression profile in mouse pre-granulosa cells alters coinciding with primordial follicle formation and oocyte development contributes to this process. Graphical Abstract
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ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioab065