A SENSITIVE, PRECISE RADIOIMMUNOASSAY OF SERUM INSULIN RELYING ON CHARCOAL SEPARATION OF BOUND AND FREE HORMONE MOIETIES

ABSTRACT A radioimmunoassay method for the measurement of plasma insulin is described relying on activated charcoal for the separation of free and bound fractions. The technique illustrates the application of theoretical precepts designed to maximise assay precision in all radioimmunoassay and other...

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Bibliographic Details
Published inActa endocrinologica (Copenhagen) Vol. 70; no. 3; pp. 487 - 509
Main Authors Albano, J D, Ekins, R P, Maritz, G, Turner, R C
Format Journal Article
LanguageEnglish
Published Denmark 01.07.1972
Subjects
Charcoal
Diabetes Mellitus, Type 1 - blood
Humans
Hypoglycemia - blood
Insulin - blood
Iodine Isotopes
Kidney Diseases - blood
Methods
Radioimmunoassay
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Summary:ABSTRACT A radioimmunoassay method for the measurement of plasma insulin is described relying on activated charcoal for the separation of free and bound fractions. The technique illustrates the application of theoretical precepts designed to maximise assay precision in all radioimmunoassay and other saturation assay techniques. In addition, because particular emphasis has been placed on ensuring that, as far as is possible, all incubation mixtures are similar as possible other than in hormone concentration, non-specific effects appear to have been essentially eliminated. The technique yields a mean normal fasting value of 5.3μU/ml (range 2–14 μU/ml). Its sensitivity is such that 10 μl samples of serum (or plasma) may be assayed.
ISSN:0804-4643
0001-5598
1479-683X
DOI:10.1530/acta.0.0700487