The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level

• Published in: Gut Vol. 67; no. 4; pp. 625 - 633
• Main Authors: Barton, Wiley, Penney, Nicholas C, Cronin, Owen, Garcia-Perez, Isabel, Molloy, Michael G, Holmes, Elaine, Shanahan, Fergus, Cotter, Paul D, O'Sullivan, Orla
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Ltd and British Society of Gastroenterology 01.04.2018; BMJ Publishing Group LTD
• Subjects: Acetic acid; Adaptation; Amino acids; Analytical chemistry; Athletes; Body Mass Index; Carbohydrate metabolism; Case-Control Studies; Chromatography; Creatine; Creatine kinase; DIET; Disease; Enzymes; Exercise; Fatty acids; Feces - microbiology; Feeding Behavior; Female; Gastrointestinal Microbiome; Gut microbiota; Humans; Intestinal microflora; Male; Mass spectrometry; Metabolism; Metabolites; Metabolomics; Microbiomes; Microbiota; MOLECULAR BIOLOGY; Phenotyping; Phylogenetics; Physical activity; Physical fitness; Physical training; Principal components analysis; Propionic acid; Scientific imaging; Sedentary behavior; Taxonomy; Urine; Young Adult; DIET; MOLECULAR BIOLOGY
• ISSN: 0017-5749; 1468-3288; 1468-3288
• DOI: 10.1136/gutjnl-2016-313627

ObjectiveIt is evident that the gut microbiota and factors that influence its composition and activity effect human metabolic, immunological and developmental processes. We previously reported that extreme physical activity with associated dietary adaptations, such as that pursued by professional athletes, is associated with changes in faecal microbial diversity and composition relative to that of individuals with a more sedentary lifestyle. Here we address the impact of these factors on the functionality/metabolic activity of the microbiota which reveals even greater separation between exercise and a more sedentary state.DesignMetabolic phenotyping and functional metagenomic analysis of the gut microbiome of professional international rugby union players (n=40) and controls (n=46) was carried out and results were correlated with lifestyle parameters and clinical measurements (eg, dietary habit and serum creatine kinase, respectively).ResultsAthletes had relative increases in pathways (eg, amino acid and antibiotic biosynthesis and carbohydrate metabolism) and faecal metabolites (eg, microbial produced short-chain fatty acids (SCFAs) acetate, propionate and butyrate) associated with enhanced muscle turnover (fitness) and overall health when compared with control groups.ConclusionsDifferences in faecal microbiota between athletes and sedentary controls show even greater separation at the metagenomic and metabolomic than at compositional levels and provide added insight into the diet–exercise–gut microbiota paradigm.