Mitochondrial DNA differences distinguishing Meloidogyne mayaguensis from the major species of tropical root-knot nematodes

• Published in: Nematology : international journal of fundamental and applied nematological research Vol. 4; no. 7; pp. 773 - 781
• Main Authors: Blok, Vivian C, Wishart, Jane, Fargette, Mireille, Berthier, Karine, Phillips, Mark
• Format: Journal Article
• Language: English
• Published: The Netherlands Brill 2002; BRILL
• Subjects: Arenaria; Biological and medical sciences; diagnostics; Fundamental and applied biological sciences. Psychology; Invertebrates; m hapla; m incognita; m javanica; Meloidogyne; meloidogyne arenaria; Nemathelminthia. Plathelmintha; Nematoda; pcr; Systematics. Geographical distribution; Taxonomy; Meloidogyne hapla; Mitochondrial DNA; Polymerase chain reaction; Meloidogyne arenaria; Meloidogyne incognita; Geographic distribution; Helmintha; Nemathelminthia; Specific identification; Molecular systematics; Invertebrata; Nematoda; Meloidogyne javanica
• ISSN: 1388-5545; 1568-5411; 1388-5545
• DOI: 10.1163/156854102760402559

Abstract - The mitochondrial DNA region between the COII and lRNA genes and the 63 base pair tandem repeat region have been used to differentiate and characterise Meloidogyne spp. In this study these regions have been amplified from M. mayaguensis, M. javanica, M. arenaria, M. incognita and M. hapla. Meloidogyne mayaguensis produces a unique product of 705 bp from the COII and lRNA region. Also, a product of 322 bp was produced from the 63 bp repeat region of M. mayaguensis unlike M. javanica, M. arenaria, and M. incognita that exhibit hypervariability in this region. Meloidogyne mayaguensis is a widely distributed root-knot nematode with the potential to cause great economic damage. These molecular diagnostics can be used for accurate identification of M. mayaguensis and can be used to monitor the occurrence and spread of this species, and to provide quarantine services tools to limit its dispersal.