Resolution of volatile fuel compound profiles from Ascocoryne sarcoides: a comparison by proton transfer reaction-mass spectrometry and solid phase microextraction gas chromatography-mass spectrometry

Volatile hydrocarbon production by Ascocoryne sacroides was studied over its growth cycle. Gas-phase compounds were measured continuously with a proton transfer reaction-mass spectrometry (PTR-MS) and at distinct time points with gas chromatography-mass spectrometry (GC-MS) using head space solid ph...

Full description

Saved in:
Bibliographic Details
Published inAMB Express Vol. 2; no. 1; p. 23
Main Authors Mallette, Natasha D, Knighton, W Berk, Strobel, Gary A, Carlson, Ross P, Peyton, Brent M
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 05.04.2012
Springer Nature B.V
BioMed Central Ltd
Springer
Subjects
Biomedical and Life Sciences
Biotechnology
Life Sciences
Microbial Genetics and Genomics
Microbiology
Original
Original Article
Solid phase microextraction
Volatile organic compounds
Biofuel
Fungal hydrocarbons
Gas chromatography-mass spectrometry
Proton transfer reaction-mass spectrometry
Online AccessGet full text

Cover

More Information
Summary:Volatile hydrocarbon production by Ascocoryne sacroides was studied over its growth cycle. Gas-phase compounds were measured continuously with a proton transfer reaction-mass spectrometry (PTR-MS) and at distinct time points with gas chromatography-mass spectrometry (GC-MS) using head space solid phase microextraction (SPME). The PTR-MS ion signal permitted temporal resolution of the volatile production while the SPME results revealed distinct compound identities. The quantitative PTR-MS results showed the volatile production was dominated by ethanol and acetaldehyde, while the concentration of the remainder of volatiles consistently reached 2,000 ppbv. The measurement of alcohols from the fungal culture by the two techniques correlated well. Notable compounds of fuel interest included nonanal, 1-octen-3-ol, 1-butanol, 3-methyl- and benzaldehyde. Abiotic comparison of the two techniques demonstrated SPME fiber bias toward higher molecular weight compounds, making quantitative efforts with SPME impractical. Together, PTR-MS and SPME GC-MS were shown as valuable tools for characterizing volatile fuel compound production from microbiological sources.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2191-0855
2191-0855
DOI:10.1186/2191-0855-2-23