Variation in the number of Bursaphelenchus xylophilus in aliquots of stock suspensions artificially inoculated on to subject pines

• Published in: Nematology : international journal of fundamental and applied nematological research Vol. 7; no. 4; pp. 623 - 630
• Main Author: Yamanobe, Taro
• Format: Journal Article
• Language: English
• Published: The Netherlands Brill 01.01.2005; BRILL
• Subjects: Agronomy. Soil science and plant productions; artificial inoculation; Biological and medical sciences; Bursaphelenchus xylophilus; COMPONENT OF VARIANCE; dilution; estimation; Fundamental and applied biological sciences. Psychology; Generalities; Genetics and breeding of economic plants; INFERENTIAL STATISTICS; inoculum; inoculum density; INTERVAL ESTIMATE; nematology; Pest animals; Pest resistance; Phytopathology. Animal pests. Plant and forest protection; pine wilt disease; PINE WOOD NEMATODE; Pinus; PRECISION; Protozoa. Invertebrates; quantitative analysis; statistical analysis; suspensions; variance; Varietal selection. Specialized plant breeding, plant breeding aims; vascular wilt; wood; component of variance; interval estimate; Pinus densiflora; precision; Artificial selection; inferential statistics; Pest; Phytophagous; Host plant; Gymnospermae; Softwood forest tree; pine wood nematode; Nematoda; Bursaphelenchus xylophilus; Estimation; Parasite; Method; Helmintha; Pest resistance; Coniferales; Nemathelminthia; Spermatophyta; Measurement accuracy; Invertebrata; Population density
• ISSN: 1388-5545; 1568-5411; 1388-5545
• DOI: 10.1163/156854105774384778

AbstractThe procedures used to estimate the number of pine wood nematodes (Bursaphelenchus xylophilus) in stock suspensions and the variation in the number of nematodes in inocula applied to subject pine trees were examined. The findings suggest that preparing dilutions using 1 ml aliquots and making two replicate dilutions and three replicate counts of each dilution (or three dilutions and two counts) results in a precision of ±15% and an elapsed time for the majority of the population estimate procedures of less than 900 seconds. Under these conditions, the variation in the number of nematodes in the inocula was estimated to range from 55 to 156% of the intended population. This variation in the number of pine wood nematodes inoculated is unavoidable; thus, researchers should account for this variation when interpreting data from trials involving the artificial inoculation of pines.