Comparison of the Efficacy of CO2-Baited and Unbaited Light Traps, Gravid Traps, Backpack Aspirators, and Sweep Net Collections for Sampling Mosquitoes Infected with Japanese Encephalitis Virus

• Published in: Journal of vector ecology Vol. 36; no. 1; pp. 68 - 74
• Main Authors: Chen, Yu-Chen, Wang, Chih-Yuan, Teng, Hwa-Jen, Chen, Chien-Fu, Chang, Mi-Chun, Lu, Liang-Chen, Lin, Cheo, Jian, Shu-Wan, Wu, Ho-Sheng
• Format: Journal Article
• Language: English
• Published: Oxford, UK Society for Vector Ecology 01.06.2011; Blackwell Publishing Ltd
• Subjects: Animals; Carbon Dioxide - pharmacology; Culex tritaeniorhynchus; Culicidae - drug effects; Culicidae - virology; Encephalitis Virus, Japanese - pathogenicity; Female; Insect Control - methods; JE virus; Light; Light traps; Male
• ISSN: 1081-1710; 1948-7134
• DOI: 10.1111/j.1948-7134.2011.00142.x

Two field studies were conducted to determine the efficacy of mosquito collection methods for species composition, species abundance, and Japanese encephalitis virus infection rates in Taiwan. Traps evaluated included John W. Hock (JH) model UD black light traps, JH model 1012 new standard miniature CDC light traps, JH model 1712 CDC gravid traps, and Taiwan-made Pest-O-Lite light traps. Backpack aspirators and sweep nets were also used to collect the resting population. Culex tritaeniorhynchus in all studies and Mansonia uniformis in the Taipei areas were the two most abundance species collected. Dry ice-baited UD black light traps were effective in regard to species diversity, species abundance, and Japanese encephalitis virus infection rates. The unbaited Pest-O-Lite light traps collected significantly more female mosquitoes than the UD black light traps but performed similarly with regard to species diversity and male mosquito collection. Most mosquitoes collected by Pest-O-Lite light traps were dried and not suitable for virus detection. Dry ice-baited CDC light traps collected significantly fewer mosquitoes than other light traps. Although CO2-baited UD black light traps with octenol attracted more mosquitoes, no statistical significance was found compared to CO2-baited UD black light traps without octenol. Japanese encephalitis viruses were isolated from half of the positive pools in UD black light traps and CDC light traps.