CULTURE MEDIUM ENHANCES SEMICARBAZIDE-SENSITIVE AMINE OXIDASE ACTIVITY

• Published in: In vitro cellular & developmental biology. Animal Vol. 38; no. 9; pp. 523 - 528
• Main Authors: TRENT, M. B, CONKLIN, D. J, BOOR, P. J
• Format: Journal Article
• Language: English
• Published: Germany Society for In Vitro Biology 01.10.2002
• Subjects: Amine Oxidase (Copper-Containing) - drug effects; Amine Oxidase (Copper-Containing) - metabolism; Amines; Animals; Aorta; Blood plasma; CELL AND TISSUE MODELS; Cell culture; Cell culture techniques; Cell Culture Techniques - methods; Cell lines; Cell Survival - drug effects; Culture Media; Cultured cells; Cytotoxicity; diabetes mellitus; Diabetic angiopathies; Endothelial cells; Enzymes; Fetal calf serum; Glucose - pharmacology; Glutamine; Glutamine - pharmacology; Hyperglycemia; Male; Media; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - enzymology; Muscles; Oxidase; Oxidases; Pyridoxine; Pyruvic acid; Rats; Rats, Sprague-Dawley; Semicarbazide; Semicarbazide-sensitive amine oxidase; Semicarbazides - pharmacology; Smooth muscle; Sodium; Sodium pyruvate; Toxicity; vascular injury; vascular smooth muscle cells
• ISSN: 1071-2690; 1543-706X; 1543-706X
• DOI: 10.1290/1071-2690(2002)038<0523:CMESAO>2.0.CO;2

Components of fetal calf serum (FCS) are known to contribute to growth and maintenance of cultured cells. Fetal calf serum supplementation of media also may contribute to the cytotoxicity of other substances to cells grown in vitro. Semicarbazide-sensitive amine oxidase (SSAO) enzyme, present in FCS, metabolizes primary amines and contributes to amine cytotoxicity in vascular smooth muscle cells (VSMC). In cell culture experiments, the media used may greatly affect enzymic activities such as SSAO. In these studies, the SSAO activity in FCS, cultured rat aortic VSMC, and rat plasma was determined in the presence and absence of various culture media. Semicarbazide-sensitive amine oxidase activity in FCS (5–20 μl) was significantly enhanced (∼1.5- to 2-fold) in the presence of various culture media, with Dulbecco modified Eagle medium (DMEM), causing the greatest enhancement. Dulbecco modified Eagle medium enhanced the SSAO activity of cultured VSMC in two of the four passages but reduced activity in two passages. Activity in rat plasma was reduced by ∼25% in the presence of DMEM. The concentrations of various media components, such as glucose, sodium pyruvate, pyridoxine·HCl, and l-glutamine, were not correlated with enhancement. This study identifies an important enhancement effect of culture media on the FCS enzyme, SSAO, although the media components responsible for the enhancement are yet to be identified.