Expression of parathyroid hormone-related protein in abnormal human parathyroids
ABSTRACT The expression of parathyroid hormone-related protein (PTHrP) in abnormal human parathyroids was investigated. Northern blot analysis of RNA extracted from human benign parathyroid adenomata (n = 4) revealed multiple PTHrP mRNA species ranging in size from 1·8 to 4kb. The relative abundance...
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Published in | Journal of endocrinology Vol. 129; no. 3; pp. 431 - 438 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Colchester
BioScientifica
01.06.1991
Portland Press |
Subjects | |
Online Access | Get full text |
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Summary: | ABSTRACT The expression of parathyroid hormone-related protein (PTHrP) in abnormal human parathyroids was investigated. Northern blot analysis of RNA extracted from human benign parathyroid adenomata (n = 4) revealed multiple PTHrP mRNA species ranging in size from 1·8 to 4kb. The relative abundance of PTHrP mRNA expressed in two of the adenomata was similar to that of a tumour (DAF) associated with humoral hypercalcaemia of malignancy, whereas PTHrP mRNA was of low abundance in a third and was undetectable in the fourth. PTHrP-like immunoreactivity was detected in extracts of abnormal parathyroid tissue (benign adenoma (n = 7), hyperplasia (n = 5) and parathyroid carcinoma (n = 2)) using a sensitive specific two-site immunoradiometric assay for human (h) PTHrP(1–86) and a radioimmunoassay for hPTHrP(1–34). Ratios of hPTHrP(1–86)- and hPTHrP(1–34)-like immunoreactivities relative to hPTH(1–84)-like immunoreactivity in the parathyroid tissue extracts were, on average, less than 1%. PTHrP bioactivity in the extracts could not be distinguished from that of PTH, by an osteosarcoma cell bioassay. We conclude that, despite reports of over-expression of PTHrP mRNA in parathyroid adenomata, the potential contribution of PTHrP to the total PTH-like activity of adenomata and other abnormal parathyroid tissue may be insignificant relative to PTH. Journal of Endocrinology (1991) 129, 431–438 |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-0795 1479-6805 |
DOI: | 10.1677/joe.0.1290431 |