Summer heat stress affects prostaglandin synthesis in the bovine oviduct

• Published in: Reproduction (Cambridge, England) Vol. 146; no. 2; pp. 103 - 110
• Main Authors: Kobayashi, Yoshihiko, Wakamiya, Kaori, Kohka, Misa, Yamamoto, Yuki, Okuda, Kiyoshi
• Format: Journal Article
• Language: English
• Published: England BioScientifica 01.08.2013
• Subjects: Abattoirs; Animals; Animals, Inbred Strains; Cattle; Cattle Diseases - metabolism; Cattle Diseases - pathology; Cells, Cultured; Cytosol - enzymology; Dinoprost - metabolism; Dinoprostone - metabolism; Enzyme Induction; Female; Heat Stress Disorders - metabolism; Heat Stress Disorders - pathology; Heat Stress Disorders - veterinary; Hot Temperature - adverse effects; HSP90 Heat-Shock Proteins - biosynthesis; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Intramolecular Oxidoreductases - biosynthesis; Intramolecular Oxidoreductases - genetics; Intramolecular Oxidoreductases - metabolism; Japan; Microsomes - enzymology; Oviducts - cytology; Oviducts - enzymology; Oviducts - metabolism; Oviducts - pathology; Prostaglandin-E Synthases; Prostaglandins - metabolism; Seasons
• ISSN: 1470-1626; 1741-7899
• DOI: 10.1530/REP-12-0479

Summer heat stress (HS) negatively affects reproductive functions, including prostaglandin (PG) F2α secretion in the endometrium, and decreases fertility in cattle. In the present study, we examined the effects of elevated temperatures on PG synthesis in oviductal epithelial cells. The epithelial cells obtained from the ampulla and isthmus of the oviduct were incubated at various temperatures (38.5, 39.5, 40.0, and 40.5 °C) for 24 h. In the ampulla, PGE2 concentration was higher at 40.5 °C than at 38.5 °C, while PGF2α production was not affected by the temperatures in this range. The expressions of microsomal PGE synthase 1 (PTGES (mPGES1)), cytosolic PGES (PTGES3 (cPGES)), and heat shock protein 90 (HSP90AA1 (HSP90)) mRNAs and proteins were higher at 40.5 °C than at 38.5 °C in the ampullary epithelial cells. Seasonal changes in the expressions of PGES and HSP90AA1 mRNAs in oviductal tissues were also investigated. The expressions of PTGES3 and HSP90AA1 mRNAs were higher in the ampullary tissues in summer than in winter. In , elevated temperatures stimulated PGE2 production in the ampullary oviduct by increasing the expressions of PGESs and HSP90AA1, which can activate cPGES. The overall results suggest that HS upsets PG secretions and reduces oviductal smooth muscle motility, which in turn could decrease gamete/embryo transport through the oviduct.