Extravascular lung water assessed by transpulmonary single thermodilution and postmortem gravimetry in sheep

• Published in: Critical care (London, England) Vol. 8; no. 6; pp. R451 - R458
• Main Authors: Kirov, Mikhail Y, Kuzkov, Vsevolod V, Kuklin, Vladimir N, Waerhaug, Kristine, Bjertnaes, Lars J
• Format: Journal Article
• Language: English
• Published: England National Library of Medicine - MEDLINE Abstracts 01.12.2004; BioMed Central Ltd; BioMed Central
• Subjects: Acute lung injury; Animals; Basic biosciences: 470; Bioinformatics: 475; Bioinformatikk; Blood Gas Analysis; Clinical medical disciplines: 750; Disease Models, Animal; Extravascular Lung Water; Extravascular lung water (EVLW); Geriatrics: 778; Humans; Inflammation; Lipopolysaccharide; Lipopolysaccharides - administration & dosage; Lung - blood supply; Lung - pathology; Lungesykdommer; Mathematics and natural science: 400; Medical disciplines: 700; Oleic acid; Oleic Acid - administration & dosage; Postmortem gravimetry; Pulmonary Artery; Pulmonary Edema - diagnosis; Pulmonary Edema - etiology; Random Allocation; Reproducibility of Results; Respiratory Distress Syndrome - physiopathology; Sheep; Single thermodilution (ST); Thermodilution - methods; VDP
• ISSN: 1364-8535; 1466-609X; 1364-8535
• DOI: 10.1186/cc2974

Acute lung injury is associated with accumulation of extravascular lung water (EVLW). The aim of the present study was to compare two methods for quantification of EVLW: transpulmonary single thermodilution (EVLWST) and postmortem gravimetric (EVLWG). Eighteen instrumented and awake sheep were randomly assigned to one of three groups. All groups received Ringer's lactate (5 ml/kg per hour intravenously). To induce lung injury of different severities, sheep received Escherichia coli lipopolysaccharide 15 ng/kg per min intravenously for 6 hours (n = 7) or oleic acid 0.06 ml/kg intravenously over 30 min (n = 7). A third group (n = 4) was subjected to sham operation. Haemodynamic variables, including EVLWST, were measured using a PiCCOplus monitor (Pulsion Medical Systems, Munich, Germany), and the last measurement of EVLWST was compared with EVLWG. At the end of experiment, values for EVLWST (mean +/- standard error) were 8.9 +/- 0.6, 11.8 +/- 1.0 and 18.2 +/- 0.9 ml/kg in the sham-operated, lipopolysaccharide and oleic acid groups, respectively (P < 0.05). The corresponding values for EVLWIG were 6.2 +/- 0.3, 7.1 +/- 0.6 and 11.8 +/- 0.7 ml/kg (P < 0.05). Ranges of EVLWIST and EVLWIG values were 7.5-21.0 and 4.9-14.5 ml/kg. Regression analysis between in vivo EVLWST and postmortem EVLWG yielded the following relation: EVLWST = 1.30 x EVLWG + 2.32 (n = 18, r = 0.85, P < 0.0001). The mean bias +/- 2 standard deviations between EVLWST and EVLWG was 4.9 +/- 5.1 ml/kg (P < 0.001). In sheep, EVLW determined using transpulmonary single thermodilution correlates closely with gravimetric measurements over a wide range of changes. However, transpulmonary single thermodilution overestimates EVLW as compared with postmortem gravimetry.