Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions

• Published in: In vitro cellular & developmental biology. Plant Vol. 44; no. 1; pp. 59 - 64
• Main Authors: Loukanina, Natalia, Thorpe, Trevor A.
• Format: Journal Article
• Language: English
• Published: New York The Society for In Vitro Biology 2008; Springer; Springer-Verlag; Springer Nature B.V
• Subjects: Acetic acid; Amino acids; arginine; auxins; Biomedical and Life Sciences; Biosynthesis; Carrots; Cell Biology; Cell culture growth; Cell culture techniques; Cell division; Cell growth; Cell lines; cell suspension culture; Comparative studies; Daucus; Daucus carota; Developmental Biology; Embryonic cells; Embryonic growth stage; Embryos; Enzymes; Life Sciences; NMR; Nuclear magnetic resonance; ornithine; Physiology; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant Sciences; Plants; Polyamine biosynthesis; Polyamines; putrescine; Putrescine formation; Somatic embryogenesis; Cell culture growth; Polyamine biosynthesis; Putrescine formation; Somatic embryogenesis
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-007-9080-3

The in vivo activities of arginine and ornithine decarboxylases, key enzymes in the biosynthesis of putrescine and thus polyamines, were measured in three different cell lines of carrot (Daucus carota) during growth and somatic embryogenesis. The activities of these two enzymes differed in the different cell lines in the presence of various levels of auxin (2,4 dichlorophenoxy acetic acid), but was highest during periods of active cell division. During somatic embryo development, the activities of both enzymes were highest during globular stage formation. Thus, both enzymes were found to be active during growth and somatic embryogenesis and could contribute to polyamine biosynthesis.