Nuclear and Cortical Regulation in Doublets of Paramecium: II. When and How do Two Cortical Domains Reorganize to One?

• Published in: The Journal of eukaryotic microbiology Vol. 48; no. 6; pp. 690 - 712
• Main Authors: IFTODE, FRANCINE, PRAJER, MALGORZATA, FRANKEL, JOSEPH
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2001; Blackwell
• Subjects: Animals; Biological and medical sciences; Cell Division; Cell Nucleus - metabolism; Cilia - genetics; Cilia - physiology; Ciliate morphogenesis; cortical development; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Life cycle. Host-agent relationship. Pathogenesis; Microscopy, Confocal; Microscopy, Electron; Morphogenesis; Paramecium - cytology; Paramecium - genetics; Paramecium - growth & development; Paramecium - ultrastructure; Paramecium doublets; Paramecium tetraurelia; Paramecium undecaurelia; pattern formation; Protozoa; unicellular development; Morphogenesis; Protozoa; Ciliary body; Immunocytochemistry; Paramecium tetraurelia; Development; Molecular rearrangement; Cytoskeleton; Ciliata; Conjugation; In vitro
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/j.1550-7408.2001.tb00210.x

Homopolar doublets with twofold rotational symmetry were generated in Paramecium tetraurelia and in P. undecaurelia by electrofusion or by arrested conjugation. These doublets underwent a complex cortical reorganization over time, which led to their reversion to singlets. This reorganization involved a reduction in number of ciliary rows, a progressive inactivation and loss of one oral meridian, and a reduction and eventual disappearance of one cortical surface (semicell) situated between the two oral meridians. The intermediate steps of this reorganization included some processes that resemble those previously described in regulating doublets of other ciliates, and others that are peculiar to members of the “P. aurelia” species-group and some of its close relatives. The former included a disappearance of one cortical landmark (a contractile vacuole meridian) and transient appearance of another (a third cytoproct) within the narrower semicell. The latter included a reorganization of the paratene zone and the associated invariant (non-duplicating) region to occupy the entire narrower semicell and a redistribution of zones of most active basal-body proliferation within the opposite, wider semicell. The final steps of reorganization involved anterior displacement, invagination, and resorption of one of the two oral apparatuses and eventual disappearance of the associated oral meridian. An oral meridian deprived of its oral apparatus, either by spontaneous resorption or microsurgical removal, could persist for some time in “incomplete doublets” before regulating to the singlet condition. The phylogenetically widespread events encountered in the regulation of doublets to singlets suggest that Paramecium shares some of the global regulatory properties that are likely to be ancestral in ciliates. The more specific events are probably associated with the complex cytoskeletal architecture of this organism and with the frequent occurrence of autogamy that was described in the preceding study (Prajer et al. 1999).