Effect of lactation on conceptus-maternal interactions at the initiation of implantation in cattle: I. Effects on the conceptus transcriptome and amino acid composition of the uterine luminal fluid

• Published in: Biology of reproduction Vol. 97; no. 6; pp. 798 - 809
• Main Authors: Forde, Niamh, Simintiras, Constantine A, Sturmey, Roger G, Graf, Alexander, Wolf, Eckhard, Blum, Helmut, Lonergan, Pat
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 01.12.2017; Oxford University Press
• Subjects: amino acid; Amino acid composition; Amino Acid Transport Systems - metabolism; Amino acids; Amino Acids - chemistry; Animals; Blastocyst; Cattle; conceptus; Corpus luteum; dairy cow; EMBRYO; Embryo Implantation; Embryo, Mammalian - metabolism; Estrous Cycle; Estrus; Female; Gene expression; High-performance liquid chromatography; Ions; Lactation; Postpartum; RNA sequencing; Sequence Analysis, RNA; Superovulation; Threonine; Transcriptome; Transcriptomes; Uterus; Uterus - physiology; Valine; RNA sequencing; dairy cow; uterus; conceptus; amino acid
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/iox135

Approximately 65–75 days postpartum (dpp), the estrous cycles of nonlactating (dried off immediately postpartum: n = 12) and lactating (n = 13) Holstein Friesian cows were synchronized and on day 7 a single blastocyst derived from superovulated nulliparous Holstein Friesian heifers was transferred to each cow. A control group of nulliparous heifers (n = 8) were synchronized, inseminated to a standing heat, and slaughtered on the same day as nonlactating and lactating recipients (day 19; estrus = day 0). The uterine horn ipsilateral to the corpus luteum was flushed with 10 ml phosphate-buffered saline and the conceptus, and uterine luminal fluid (ULF) was snap-frozen in liquid nitrogen. Gene expression analysis of the conceptus was performed by RNA sequencing, while amino acid composition of ULF was determined by high-performance liquid chromatography. No differentially expressed genes (DEGs) were observed between conceptuses recovered from nonlactating and lactating cows. Eight DEGs were identified between conceptuses recovered from nonlactating cows and heifers. A total of 269 DEGs (100 up- and 169 downregulated) were identified between conceptuses recovered from lactating cows compared to heifers. Alanine, glycine, serine, threonine, arginine, leucine, and valine were significantly lower in abundance in ULF recovered from heifers compared to nonlactating or lactating cows. This study demonstrates that the environment in which the embryo develops post the blastocyst stage can have an effect on the conceptus transcriptome and amino acid composition of the ULF but this was mainly observed between the two extreme groups in terms of metabolic status (nulliparous heifers vs postpartum lactating cows). Summary Sentence Exposure of embryos from a common pool to a uterine environment under different metabolic statuses alters conceptus transcriptome and composition of uterine fluidmainly between extremes of metabolic status.