A plant-defensin from sugarcane (Saccharum spp.)

Comparing available Poaceae defensins with sugarcane ESTs, a putative defensin gene was identified in sugarcane and cloned from genomic sugarcane DNA. The deduced encoded peptide shows the structure and amino acid composition typical of other plant defensins. Using RT-PCR, defensin expression in sug...

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Bibliographic Details
Published inProtein and peptide letters Vol. 16; no. 4; p. 430
Main Authors Padovan, Lara, Segat, Ludovica, Tossi, Alessandro, Antcheva, Nikolinka, Benko-Iseppon, Ana Maria, Ederson, Akio Kido, Brandao, Lucas, Calsa, Jr, Tercilio, Crovella, Sergio
Format Journal Article
LanguageEnglish
Published Netherlands 01.04.2009
Subjects
Amino Acid Sequence
Base Sequence
Defensins - chemistry
Defensins - isolation & purification
DNA, Plant - genetics
Expressed Sequence Tags
Molecular Sequence Data
Plant Proteins - genetics
Saccharum - genetics
Saccharum - metabolism
Sequence Alignment
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Summary:Comparing available Poaceae defensins with sugarcane ESTs, a putative defensin gene was identified in sugarcane and cloned from genomic sugarcane DNA. The deduced encoded peptide shows the structure and amino acid composition typical of other plant defensins. Using RT-PCR, defensin expression in sugarcane and differences between "normal" and infected sugarcane were evidenced.
ISSN:0929-8665
DOI:10.2174/092986609787848027