Differentially expressed lncRNA-m433s1 regulates FSH secretion by functioning as a miRNA sponge in male rat anterior pituitary cells

• Published in: Biology of reproduction Vol. 101; no. 2; pp. 416 - 425
• Main Authors: Han, Dong-Xu, Sun, Xu-Lei, Wang, Chang-Jiang, Yu, Ze-Wen, Zheng, Yi, Huang, Yi-Jie, Wang, Wen-Hua, Jiang, Hao, Gao, Yan, Yuan, Bao, Zhang, Jia-Bao
• Format: Journal Article
• Language: English
• Published: United States Society for the Study of Reproduction 01.08.2019; Oxford University Press
• Subjects: Animal reproduction; Animals; Anopheles; Apoptosis; Cell culture; Cell cycle; Female; Follicle Stimulating Hormone, beta Subunit - genetics; Follicle Stimulating Hormone, beta Subunit - metabolism; Follicle-stimulating hormone; FSH; Gene expression; Gene Expression Regulation - physiology; Hormones; Hybridization; Laboratory animals; lncRNA; Male; Males; Methods; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA sponge; Novels; Physiological aspects; pituitary; Pituitary Gland, Anterior - cytology; Pituitary hormones; Rats; Rats, Sprague-Dawley; RNA; RNA sequencing; RNA, Long Noncoding - genetics; Rodents; Thyroid gland; Up-Regulation; United States; pituitary; miRNA sponge; FSH; lncRNA; animal reproduction
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1093/biolre/ioz100

Long noncoding RNAs (lncRNAs) are important regulators that have multiple functions in a variety of biological processes. However, the contributions of lncRNAs to follicle-stimulating hormone (FSH) secretion remain largely unknown. In this study, we first identified a novel lncRNA, lncRNAm433s1, as an intergenic lncRNA located in the cytoplasm. We next used MS2-RIP assays to demonstrate that lncRNA-m433s1 interacted with miR-433. Furthermore, we detected the levels of lncRNA-m433s1, miR-433, and Fshβ expression, FSH concentrations, and apoptosis upon overexpression and knockdown of lncRNA-m433s1, revealing that lncRNA-m433s1 upregulated Fshβ expression. Globally, lncRNA-m433s1 reduced the inhibitory effect of miR-433 on Fshβ and further regulated FSH secretion as a competing endogenous RNA (ceRNA) by sponging miR-433. This ceRNA model will provide novel insight into the regulatory mechanisms of lncRNAs associated with rat reproduction. Summary Sentence A regulatory mechanism of lncRNA in rat anterior pituitary cell which was that lncRNA-m433s1 reduced the inhibitory effect of miR-433 on Fshβ and further regulated FSH secretion by acting as a ceRNA to sponge miR-433.