A NEW PARVICAPSULID (MYXOSPOREA) SPECIES IN ADULT PINK SALMON, ONCORHYNCHUS GORBUSCHA, FROM THE QUINSAM RIVER, BRITISH COLUMBIA, CANADA

• Published in: The Journal of parasitology Vol. 92; no. 6; pp. 1313 - 1318
• Main Authors: Jones, Simon, Prosperi-Porta, Gina, Dawe, Sheila
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01.12.2006
• Subjects: Animal and plant ecology; animal parasites and pests; Animal, plant and microbial ecology; Animals; Base Sequence; Biological and medical sciences; Brackish; British Columbia - epidemiology; Capsules; Cloning, Molecular; DNA, Protozoan - chemistry; DNA, Ribosomal - chemistry; Eukaryota - classification; Eukaryota - genetics; Eukaryota - ultrastructure; fish; fish diseases; Fish Diseases - epidemiology; Fish Diseases - parasitology; Fresh water ecosystems; Freshwater; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Histology; Infections; Invertebrates; Kidney - parasitology; Kidneys; Loma salmonae; Marine; Molecular Sequence Data; molecular systematics; Myxidium; Myxosporea; myxospores; Myxozoa; new species; nucleotide sequences; Oncorhynchus; Oncorhynchus gorbuscha; Oncorhynchus kisutch; Parasites; Parasitology; Parvicapsula; Parvicapsula kabatai; Parvicapsula pseudobranchicola; Phylogeny; Polymerase Chain Reaction - veterinary; Prevalence; protozoal infections; Protozoan Infections, Animal - epidemiology; Protozoan Infections, Animal - parasitology; Renal tubules; Ribosomal DNA; Rivers; RNA, Protozoan - genetics; RNA, Ribosomal - genetics; Salmo salar; Salmon; Salmon - parasitology; spore morphology; Spores; Spores, Protozoan - ultrastructure; Synecology; SYSTEMATICS-PHYLOGENETICS; taxonomy; ultrastructure; British Columbia; Canada; North America; America; Canada, British Columbia, Vancouver I., Quinsam R; INE, USA, Washington, Puget Sound; Freshwater environment; Vertebrata; Taxonomy; Pisces; Diagnosis (taxonomy); Parasite; Stream; New species; Oncorhynchus gorbuscha
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/GE-940R.1

Myxospores consistent with species of Parvicapsula were observed in kidney of 15 of 95 (15.8%) adult pink salmon, Oncorhynchus gorbuscha, collected from the Quinsam River, British Columbia, Canada. The spores were elongate and curved with unequal valves, and 2 spherical-to-subspherical polar capsules within a highly refractile capsular region. The spores were unlike those of P. minibicornis found in nearby populations of Pacific salmon, Oncorhynchus spp. The spore dimensions were similar to those of Parvicapsula pseudobranchicola from Atlantic salmon, Salmo salar, in Norway, and the spores seemed similar to an undescribed Parvicapsula sp. from several Oncorhynchus spp. in Puget Sound, Washington. A sequence of 1,480 base pairs (bp) of the small subunit ribosomal RNA gene (SSU rDNA) of the parasite from pink salmon was most similar to, but distinct from, that of other Parvicapsula spp. The parasite is described as a new species, Parvicapsula kabatai n. sp. Polymerase chain reactions amplified a 158-bp sequence, unique to P. kabatai n. sp., from 22 of 93 (23.7%) adult pink salmon kidney samples, from 3 of 3 juvenile pink salmon collected in the ocean 125 km north of the Quinsam River, and from 2 of 5 archival coho salmon, Oncorhynchus kisutch, samples from Puget Sound. The parasite occurs within the lumen and epithelium of renal tubules and ducts, and within the renal interstitium. Concurrent infections with extrasporogonic stages of the myxosporean CKX, the microsporidian Loma salmonae, and a Myxidium sp. also were observed in the adult pink salmon.