An improved assay to quantitate the invasiveness of cells in modified Boyden chambers

The most commonly used means of assessing the invasiveness of cultured cells is the Boyden chamber assay, which requires that cells lyse Matrigel™, followed by migration through pores in a filter in response to a chemotactic gradient. This report describes a simple method, which greatly increases th...

Full description

Saved in:
Bibliographic Details
Published inIn vitro cellular & developmental biology. Animal Vol. 43; no. 1; pp. 7 - 9
Main Authors Woo, Michelle M. M., Salamanca, Clara M., Minor, Agata, Auersperg, Nelly
Format Journal Article
LanguageEnglish
Published Germany The Society for In Vitro Biology 01.01.2007
Springer Science + Business Media
Society for In Vitro Biology
Subjects
Boyden chamber
Cancer
Cell Count
Cell culture techniques
Cell nucleus
Chemotaxis
Cultured cells
Cytological Techniques - methods
Endothelial cells
Epithelial cells
Image analysis
Invasion assay
Invasion in vitro
Keratins
Micropore Filters
Stromal cells
Subpopulations
Tumor Cells, Cultured - cytology
Tumors
Online AccessGet full text

Cover

More Information
Summary:The most commonly used means of assessing the invasiveness of cultured cells is the Boyden chamber assay, which requires that cells lyse Matrigel™, followed by migration through pores in a filter in response to a chemotactic gradient. This report describes a simple method, which greatly increases the speed and accuracy by which Boyden chamber assays can be analyzed, and permits the concurrent analysis of distinct cell subpopulations within specimens containing multiple-cell types.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-006-9002-4