Transcriptional regulation of expression of the rainbow trout albumin gene by estrogen

Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene...

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Published inJournal of molecular endocrinology Vol. 20; no. 3; pp. 355 - 362
Main Authors Flouriot, G, Ducouret, B, Byrnes, L, Valotaire, Y
Format Journal Article
LanguageEnglish
Published England BioScientifica 01.06.1998
Subjects
Animals
Cell Nucleus - metabolism
Cells, Cultured
Estradiol - pharmacology
Gene Expression Regulation - drug effects
Kinetics
Liver - cytology
Liver - drug effects
Liver - metabolism
Male
Oncorhynchus mykiss
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Serum Albumin - biosynthesis
Serum Albumin - genetics
Time Factors
Transcription, Genetic - drug effects
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Summary:Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 microM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run-off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0952-5041
1479-6813
DOI:10.1677/jme.0.0200355